Katie Galloway
@GallowayLabMIT
Assistant Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo
Delighted to bring gene circuits into a wide array of primary cells where they can be used for safe, effective gene therapies. 🧵 (1/n) Tremendous work by lead author @kaseyslove to dig into the details of these systems and figure out how to get these to work well! Original…
Loss of gene expression can compromise cellular fitness. In the case of such haploinsufficiency, replacement of the gene supplementation could rescue cell health. But what if you need a very precise dosage? Try ComMAND! 🧵 (1/n) Link: doi.org/10.1101/2024.0…
senior grad student plasmid prepping for fig S36F
Humans can get so good at weird manual dexterity shit this why biotech R&D is hard to automate
Nifty! This can solve a lot of challenges we see with DNA delivery! Nice work @GraceKC!
Can we use RNA to insert kilobases of DNA into the human genome, opening up a new way to engineer cells or treat disease? Excited to share our latest work turning a retrotransposon (a “jumping gene” with an RNA intermediate) from songbirds into a genome editing tool. 🧵 (1/7)
New preprint! We solve a mystery you didn't know existed. Mitotic cells lack new transcription but require ongoing translation. Interphase mRNA half life is only 2-4 hrs. So how do cells arrest in mitosis for hours without depleting their transcriptomes? biorxiv.org/content/10.110…
What!?!
NIH will only accept six applications per PI per calendar year. drugmonkey.wordpress.com/2025/07/17/nih… via @drugmonkeyblog
More than 2700 3′UTRs are highly conserved. These 3′UTRs are essential components in mRNA templates, as their deletion decreases protein activity without changing protein abundance. Highly conserved 3′UTRs help the folding of proteins with long IDRs. biorxiv.org/content/10.110…
📊 La regresión lineal parece sencilla… hasta que deja de serlo. 💥 ¿Estás seguro de que la estás aplicando bien? Aquí van 3 riesgos clave que deberías tener bajo control para no sacar conclusiones equivocadas. 👇 #stats #analytics #DataScience #AnálisisDeDatos #Estadística
until you defEND
boyfriEND. girlfriEND. bestfriEND. you know what doesn't end? A PhD
Happy Independence Day! 🇺🇸 #Happy4thofJuly #Happy4th #independenceday2025
New from the Rouhanifard Lab! What if we could sort single cells based on whether a specific gene was actively being transcribed—and then ask what drives that burst? Introducing: NuclampFISH
The ISSCR has selected five distinguished early career scientists to serve as new Early Career Editors for Stem Cell Reports, the peer-reviewed, open access, online journal of the International Society for Stem Cell Research (ISSCR). Learn more: ow.ly/hfGs50Wk0il
Poster abstract submissions for #SBA2025 are closing on 18th July - get yours in now! See you in Wellington in November #syntheticbiology #synbio #conference @SynBioAusAsia
Watching people argue about models or experiments as essential features in biology is like watching people argue if a car should have brakes or a gas pedal. You probably drive most effectively if you can use both.
probably one of the cooler boundary collisions this year just now in the TX Panhandle #TXwx
Kid 1: who's the best actor? Jim Carrey? Kid 2: what about Charles Dickens? Me: Dickens is a writer. Kid 3: Dickens isn't a writer. Me: Yes, he is. Kid 3: No, He's dead. His profession is being dead. He has years of experience....He has tenure. 💀😂
high-throughput single-molecule platform to study DNA supercoiling effect on protein–DNA interactions academic.oup.com/nar/article/53…
Ah, here is the working link to the paper. Not sure why the original link doesn't work. academic.oup.com/nar/article/53…
So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening? [1/n] Well, promoters don’t just set RNA levels; they uniquely transform how RNAs are transmitted into protein…