Sara Rouhanifard
@SRouhanifard
Assoc. Prof of bioengineering @Northeastern University | RNA modifications | RNA localization | click chemistry | single-molecule studies
🎉 Our new paper on live, intracellular #ClickChemistry just posted in @NatureComms! Huge shoutout to lead author @KeqingN for all of the hard work 🙌🏼 Check it out 👉🏼 rdcu.be/enfHz
A major technological advancement that opens up an entirely new area of cell biology ... @nature reports a versatile platform for high-throughput functional interrogation of RNA localization in living cells and organisms. doi.org/10.1038/s41586…
Hello RNA World! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @CellReports, @FKHM and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit! tinyurl.com/ydn6e3ac
So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening? [1/n] Well, promoters don’t just set RNA levels; they uniquely transform how RNAs are transmitted into protein…
Cells are dynamic, messy and context dependent. Scaling models across diverse states needs flexibility to capture heterogeneity Introducing State, a transformer that predicts perturbation effects by training over sets of cells Team effort led by the unstoppable @abhinadduri
New online! RNA polymerase II transcription compartments — from factories to condensates bit.ly/44osaMv
If you think you’re tough, consider the tardigrade… In 2007, 3,000 of these tiny animals orbited the Earth OUTSIDE a space rocket for 12 days. >2/3 of them survived the vacuum of space and being blasted with solar radiation. esa.int/Science_Explor…
A new Perspective by Mark Howarth discusses click biology as an analogy to click chemistry, examines reactions using building blocks present in living cells, and highlights opportunities for fundamental research and synthetic biology nature.com/articles/s4158…
Plasmid-based reporter assays are the bedrock of regulatory genomics. But a basic question has gone unanswered for decades: Do chromatin architectures form on plasmids transfected into mammalian cells—and does it matter? We finally have answers. biorxiv.org/content/10.110…
New paper from my group rdcu.be/eoaRr "Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme" in Nature Chemistry — great collaboration with attwaterlab.uk.