Nicholas Perry
@ntperry13
Scientist, Hsu Lab UCB-UCSF Bioengineering PhD Arc Institute Genome design and bridge recombinases
"Does it work in human cells?" is a question I've gotten a lot this past year. Excited to share our latest work on bridge recombinases! It's been a lot of fun to lead this team effort. If you are excited to hear more, join for my thesis defense tomorrow at 3pm PT. Links below:
Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases In new @arcinstitute work, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
Fantastic news for genome engineers: Bridge recombinases work in human cells!!🧬 And they can make really big insertions, deletions, and inversions with impressive efficiency. From @arcinstitute @pdhsu @ntperry13 & team
In the ocean, a single-celled organism travels ~50 meters vertically by rapidly inflating its body with water. The dinoflagellate, Pyrocystis noctiluca, does this by swelling six-fold in size in just ten minutes. This fast expansion drops cell density. When the cell becomes less…
The Case for Bridge Editors Many people seem confused about the gene-editing tool released by @arcinstitute. How does it work and why does it matter? Fair questions. The preprint is not easy to understand, and the reasons for inventing a new gene-editing tool are not obvious...…
Big deal: Bridge recombinases are now working in human cells. Preprint here: biorxiv.org/content/10.110…
Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases In new @arcinstitute work, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement
We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their…
me with deepresearch: “find obscure biochemistry papers from the 1960s and 70s that have very weird but intriguing results, and there have been no follow up experiments since”
Congratulations Alison! Looking forward to seeing what people do with your engineered integrases in stem cells and T cells. Check out @AlisonFanton exciting thesis work here: biorxiv.org/content/10.110… nature.com/articles/s4158…
Super proud of the fearless @AlisonFanton for her PhD defense yesterday! Alison is a super talented bioengineer and molecular biologist who has done pioneering work on DNA recombinases for human genome insertion. Congratulations, Dr. Fanton!
Hey folks, I know most deadlines are fast approaching, but I am applying for assistant professor positions this cycle, and I would appreciate recommendations for open positions! My research plan is focused on MGEs, genome engineering, and applying AI to biological discovery.
A recent & exciting scientific discovery is Bridge RNA - a new class of guide RNAs that could recombine, insert, delete, or invert any 2 pieces of DNA. Discovered by @pdhsu, @mgdurrant & @ntperry13 - here is a great video by @arcinstitute which explains this new modality.
Did you see the bridge RNA papers yesterday? Bacterial transposons promote programmable, RNA-guided DNA recombination. Remarkably, we found that the recombinase was co-opted in archaea and eukaryotes for widspread RNA-guided RNA modification, an essential function in humans!
New preprint: In a fun collaboration with the @SternbergLab, we reveal the evolutionary origin of eukaryotic RNA-guided RNA modification (Nop5 and Prp31) in recently characterized bacterial IS110-family transposons. biorxiv.org/content/10.110…
On the topic of RNA-guided biology emerging from transposons, you won't want to miss this one! In published work led by the talented @TannerWiegand, we uncovered the presence of pervasive, RNA-guided transcription factors in diverse biological contexts. nature.com/articles/s4158…
Check out the latest story from the Sternberg group: phages coopted transposon-encoded nucleases (TnpB) and repurposed them for regulation of host genes! nature.com/articles/s4158… Thrilled to see this work in print, & a huge thanks to my amazing co-authors!!
Congrats to @mgdurrant @ntperry13 @pdhsu @hnisimasu and colleagues on their bridge recombinase works! Excited to see how this technology progresses. 🧬 @BKleinstiver and I highlight their two papers in @Nature @NatureNV: nature.com/articles/d4158…
This morning, Nature published two papers on bridge editing, the new genome engineering technology from @ArcInstitute: nature.com/articles/s4158…, nature.com/articles/s4158…. I'm quite excited about its potential! Since the whole thing is pretty arcane, I fed the blog post…