Lucas Waldburger

Today the Shih Lab (@LabShih) presents ENTRAP-seq, a high-throughput assay for functional screening directly in plant cells.

Check out our new preprint! We examined sources of variability of transgene expression in N. benthamiana agroinfiltration and ways to minimize it. Graduate student Sophia Tang observed FP expression in >1,800 plants over the course of nearly 3 years! biorxiv.org/content/10.110…

Happy to share our new publication out today in @NaturePlants where we quantitatively dissect synergy and antagonism that occurs during Nicotiana benthamiana transient agroinfiltration. Congrats to Simon, Matthew, Mitch and all other co-authors! nature.com/articles/s4147…

New OA Article: "Quantitative dissection of Agrobacterium T-DNA expression in single plant cells reveals density-dependent synergy and antagonism" rdcu.be/elJ5z News & Views: "Cooperation and antagonism in Agrobacterium-mediated transformation" rdcu.be/elJ5F

Excited to share our new CRAGE-based method for studying root-colonizing bacteria🌱🦠 This approach enabled us to profile thousands of promoter activities in Pseudomonas simiae during Arabidopsis root colonization, overcoming plant RNA interference. biorxiv.org/content/10.110…

New research shows that some simple changes to Agrobacterium can significantly improve the efficiency of introducing DNA into a genome, also known as “transformation.” @JBEI @igisci newscenter.lbl.gov/2024/11/07/new…

I am pleased to announce new research from the Shih Lab (@LabShih) published today in Nature Biotechnology on improving Agrobacterium-mediated transformation via binary vector copy number engineering (doi.org/10.1038/s41587…). 🧵A summary thread below🌿

Binary vector copy number engineering improves Agrobacterium-mediated transformation go.nature.com/3CdewQS

The Keasling Lab at UC Berkeley/LBNL is looking for motivated undergraduate students interested in applying machine learning in metabolic engineering and fungal/plant synthetic biology. Apply here: 👇

The Hill-Maini lab website is live @bioe_stanford @Stanford. We are hiring for multiple postdocs who are excited about synbio/food/sustainability in filamentous fungi and building an inclusive and lab culture that fosters creativity! Spread the word!!! hillmainilab.com

Only reason I see for authors to use biosecurity as an excuse to limit access to source code/weights is to gain advantage over their competition.

Our work, led by former postdoc @Liulabscripps and Professor @jaykeasling, on the complete biosynthesis of QS-21 in engineered yeast is out now in @Nature. We report the sustainable supply chain of an essential adjuvant for global immunization here: nature.com/articles/s4158…

➡️ Enjoy this short piece with @v_courdavault highlighting the pionnering acheivements by the @Keasling_Lab on metabolic engineering of plant medicines 🌿💊 nature.com/articles/s4157… @NatureRevGenet @BBVEA2106_Tours @UnivAngers @UA_Recherche @chu_angers @FacSante_Angers @icat_sfr

Really cool to see this work out on minimal Ti plasmids to better understand Agrobacterium-mediated transformation of plants and fungi.

Very happy to have the big work of my postdoc in .@LabShih and .@jbei out to the public. We have successfully minimized and refactored Agrobacterium mediated transformation into a synthetic vector 1/n biorxiv.org/content/10.110…