DanPiraner
@piraner
I engineer proteins, cells, and puns.
Cute story alert!🧵@Caltech two clueless office mates (@piraner & I) created a tool to control cells with ultrasound. As postdocs (@UCSF & @UWproteindesign), we teamed up again (with @majo__duran & @AdamChazin) to build a tool for engineered cell signaling nature.com/articles/s4158…
Why test #celltherapies one-by-one when you can use #SynBio to design, learn, and iterate on thousands in parallel? Our team @UCSF @MarsonLab @roybal_lab is #hiring a wetlab technician to help build the next revolution in genetically engineered medicine! DM for info & please RT!
Congrats Avinoam and team! Very cool work showing how ultrasound can synergize with synthetic biology to blast away at tumors. This was definitely one of the most fun projects to hear about in the office!
Can cells be engineered to infiltrate a tumor and blow it up from the inside? Yes, when they express #gasvesicles as seeds for ultrasound-triggered bubble cavitation! Congrats to star postdoc @bar_zion and team on this publication! nature.com/articles/s4156…
1/5 I am excited to share this pre-print of ours out yesterday. Really fun project with @MaxKrummel and @KoleRoybal digging into CAR T cell synapses and how variations in binding affinity/avidity affect synapse dynamics and T cell triggering. biorxiv.org/content/10.110…
I'm late to the tweeting party, but if you haven't had a chance, check out the latest preprint from the Roybal Lab! The team, led by Dan Goodman and Camillia Azimi used pooled screening to find CARs that induce augmented phenotypes in T-cells! Congrats Dan, Camillia, and Team!
Excited to share new work w/ co-lead @CamilliaAzimi, @MarsonLab @kickassscience1 @roybal_lab: using a method we call 'CAR Pooling', we tested many CARs in multiplex & found new non-T cell signaling domains which improve CAR-T efficacy. biorxiv.org/cgi/content/sh… 👇🧵 1/10
Excited to share new work w/ co-lead @CamilliaAzimi, @MarsonLab @kickassscience1 @roybal_lab: using a method we call 'CAR Pooling', we tested many CARs in multiplex & found new non-T cell signaling domains which improve CAR-T efficacy. biorxiv.org/cgi/content/sh… 👇🧵 1/10
If you're interested in therapeutic SynBio and haven't seen these papers yet, check them out! Fantastic work from the Roybal and Lim labs demonstrating the enhanced precision and efficacy of engineered logic-gated T-cells!
1/4 We’re excited to share two major papers out @ScienceTM showing the many benefits of synNotch CAR circuits for the treatment of solid tumors. Axel Hyrenius-Wittsten, an incredible postdoc @roybal_lab, led the studies in Ovarian Cancer and Mesothelioma. tinyurl.com/drje876u
Exciting work by @Mohamad_Abedi and team to engineer a robust bi-stable system that actuates over a very small and therapeutically relevant temperature shift, along with a great demonstration of what on-demand in situ drug production can do.
Want a cancer cell therapy? would like to avoid bankruptcy* ? Engineered bacteria holds the promise for a cheap therapy that can penetrate deep into immunosuppressive tumors (where T cells are excluded) *only applies in the USA biorxiv.org/content/10.110…
Can tumor-infiltrating bacterial immunotherapy be controlled with focused ultrasound to enhance its specificity? Congratulations to @Mohamad_Abedi, @michael_s_yao and colleagues @Caltech on demonstrating this possibility in their new bioRxiv preprint. biorxiv.org/content/10.110…
1/ Synbio has potential to enhance next-gen therapies, but how can we make clinically-viable, therapeutic circuits in human cells a reality? Excited to unveil 1st of several papers trying to address this question, led by the brilliant @phdivya & Huishan Li biorxiv.org/content/10.110…