Maria del Mar Ingaramo
@Maria_Ingaramo
I'm a biochemist. I develop new fluorescent proteins and biosensors.
I really enjoyed this project, my first time combining biochemistry and invention, to try to make a new kind of microscope! It set the stage for the type of scientist I've grown into.
Hey! IMHO, the non-linear version of Airyscan (or multifocal SIM or iSIM) was first demonstrated by Ingaramo, @AndrewGYork et al for a quadratic non linearity. nature.com/articles/ncomm…
Here's why our beautiful 🐸 paper is not about 🐸s and not about embryogenesis---a 🧵 biorxiv.org/content/10.110…
MagLOV results reproduced✅ We cranked the LED power and found the response can be much faster! Absolute change roughly the same at each LED power, i.e. contrast change likely due to the background, not MagLOV response. Many thanks to the Calico folks for sharing their discovery!
Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields. This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate. Can you tell where the magnet is? Want some? It's on Addgene now! addgene.org/219957/
Hey magnetogeneticists! I'll be presenting our new results on a highly magnetic-field-sensitive AsLOV2 variant at the Big Quantum Bio meeting tomorrow Thursday, April 18, at 7 am PST/10 am EST/3 pm UK time @ groups.google.com/g/bigquantumbi…. See you there!
This is a cool system for evolving optogenetic tools, and it makes a surprising red LOV protein to prove it!
Can you evolve proteins that switch, compute, or have multiple states in the lab? The brilliant Vojislav Gligorovski (@glig_v) came up with a way to do it: Create a cascade where optogenetics controls your protein of interest, which in turn controls cell cycle oscillations.(1/2)
The uropod of the amoeba Chaos is labeled by microinjecting Lifeact-GFP2 protein. The #AIRR Single Objective Light Sheet by @amsikking and the team at Calico is a game changer for depth and speed! We imaged approx. 250x320x100um^3 volumes in <1s using a 40x 0.95 NA objective.
More about the miRFP photo-conversion in the NIR to red spectral region!
Excited to share our latest research where we unveiled a photoconversion mechanism entirely in the near infrared (NIR) within the miRFPs family, advancing photolabeling studies in vivo and expanding photoconversion multiplexing. nature.com/articles/s4146…
Our scientists are using advanced technologies like #microscopy to visualize never-before seen aspects of biology. Learn more about why Kayley and her colleagues find Life at Calico so rewarding: bit.ly/3ScFDBn
Today, we are announcing Future House, a philanthropically-funded moonshot focused on building an AI Scientist. At Future House, our 10-year mission is to build semi-autonomous AIs for scientific research, to accelerate the pace of discovery and to provide world-wide access to…
A thought-provoking, page-turner exploration of a really cool technique that we are working on, brought to you by my favorite scientist/science writer of all times @JLazzariDean
Molecular interactions are essential for life, but they’re invisible to our microscopes. But some confocals are a software upgrade away from revealing binding: andrewgyork.github.io/tumbling_is_ge… Imagine cytometers & lightsheets that reveal binding partners of GFP-labeled proteins, live!
I'm really proud of this work. Truly looking forward to see how much better we can make it, and what technology it unlocks.
We discovered a surprisingly easy way to control the brightness of fluorescent proteins via magnetic fields: andrewgyork.github.io/gfp_magnetoflu… This raises fascinating scientific questions about the mechanism, and tantalizing technological opportunities for bioengineering.
A month ago, there wasn’t a general tool to measure protein interactions in live cells. It certainly wasn’t for lack of trying. If you’re thinking “but FRET! colocalization! FCS!”, read on! @AndreaVolpato @IlariaTesta4 et al changed this nature.com/articles/s4158… Here’s my take:
So useful and what a fantastic delivery! 'Monsieur Poisson is as dim as he should be'!?! And I am very glad that the Babylonians got their well-deserved credit.
Shameless advertising: #FOM2022 Wednesday, Various Topics III, 13:25–13:40 (CEST, UTC+2): I will present why you might not want to do the image processing operation in the video below. Also, the talk immediately beforehand is much cooler!
Love this type of work that goes back to the fundamentals! It reminds you that we forgot that we did not know it all.
Turning a scallop into a #Microscope: For everyone who could not attend my @FOMconferences #FOM2022 talk and is curious about how to build multi-immersion objectives combining large FOVs, large NAs, and long working distances: Here is the Youtube version: youtube.com/watch?v=Jmi6pT…
If you are interested in lysosomal biology, check out this super-sturdy pH sensor from @JLazzariDean. Do reach out if you want to try it out; we'd be stoked to help you give it a whirl!
I made a new quantitative pH sensor for lysosomes w/@Maria_Ingaramo! You may ask: aren’t there already tons of pH sensors? Yes, but this one is SUPERB for lysosomes: bright, quantitative, targetable & single-color. mScarlet lifetime reports lysosomal pH: andrewgyork.github.io/mScarlet_lifet…
Interested in understanding and making new fluorescent biosensors? Come be part of this collaboration between the Tim Craggs lab @Craggs_lab and the Calico microscopy team! @andrewgyork @JLazzariDean
Apply now for a new collaborative #PhD project between @Craggs_lab and Calico! Would you like to use cutting-edge biophysical methods to characterise a new class of biosensors developed by @Maria_Ingaramo and @AndrewGYork? Apply here: bit.ly/3jaOkK0 or DM for more info.
Beautiful story. I always fill with emotion hearing personal stories about the titans of the field. It brings another dimension to the science that we spend so much time with.
Seeing this imaging innovation research tweet from @Maria_Ingaramo @AndrewGYork reminded me of a story about meeting Professor #RogerTsien. I usually don't tweet stories, but I was reminded of this one today. So here we go. 🧵
This. is. incredible! @Maria_Ingaramo is a magician. Check out the story of how she invented a spontaneously relaxing biosensor that allows for quantitative organelle/tissue specific measurements in whole animals & leverages its properties to circumvent autofluorescence 🤯
Fluorescent biosensors reveal the dynamics of life, but *quantitative* biosensing only works in transparent single cells... until now! andrewgyork.github.io/relaxation_sen… @Maria_Ingaramo figured out how to take robust quantitative measurements with fluorescent biosensors, in whole animals.
Fluorescent biosensors reveal the dynamics of life, but *quantitative* biosensing only works in transparent single cells... until now! andrewgyork.github.io/relaxation_sen… @Maria_Ingaramo figured out how to take robust quantitative measurements with fluorescent biosensors, in whole animals.