Jason Buenrostro

I’m so honored and grateful to be named a MacArthur Fellow! I’ve been absolutely flooded with texts, emails and likes. What a wonderful experience to hear from colleagues, collaborators, friends, and family. Thank you all for the support and encouragement!

Unveiling Expansion in situ genome sequencing: a revolutionary leap in spatial genomics! ExIGS enables simultaneous nanoscale mapping of gDNA and proteins in single cells. It's been an incredible journey co-leading this work with @z_chiang , @CarolineComenho , @JD_Buenrostro

It's a wrap on my postdoc @Harvard. It's been a privilege to develop ExIGS and contribute to the birth of expansion genomics! Deeply grateful to @JD_Buenrostro, my amazing co-authors @z_chiang & @CarolineComenho, & colleagues who made Boston feel like home. Goodbye!

🔬 The @JD_Buenrostro lab at the @broadinstitute has developed a technology that provides new insight into how disruptions in the nucleus of the cell can impact health and disease. Learn more about expansion in situ genome sequencing: bit.ly/4437mdc

A new combination technique enables DNA sequencing and high-resolution imaging in intact cells — offering new insights into progeria and aging. broad.io/expansion-in-s… @JD_Buenrostro @insitubiology @ajaylabade31 @CarolineComenho @z_chiang @ScienceMagazine

Expansion in situ genome sequencing links nuclear abnormalities to aberrant chromatin regulation science.org/doi/10.1126/sc… @ScienceMagazine @JD_Buenrostro @ajaylabade31 @CarolineComenho

The peer-reviewed version of expansion in situ genome sequencing is now out in Science! The news is bittersweet – when we first revealed this last September, I never guessed it would be my final paper in academia, but a lot has changed. A few parting thoughts:

Thrilled to announce that our work on Expansion In Situ Genome Sequencing (ExIGS) is now published in @ScienceMagazine! ExIGS allows us to sequence DNA and image proteins with super-resolution directly within single cells. science.org/doi/10.1126/sc…

We’d like to thank our collaborators in the @ohyilmaz and Breault labs, and PROSPECT @CR_UK @theNCI for their support. Find out about binding partners, drugging AP-1, the new tools we made and more here at our pre-print: tinyurl.com/ColitisNagaraj…

I’m excited to share my work over the last few years with @JD_Buenrostro! Chronic inflammation creates an epigenetic memory in colonic stem cells, priming them for tumor growth: tinyurl.com/ColitisNagaraj…. Here’s a walkthrough of what we found (🧵)

“Biology and AI form a two-way street—the diverse expertise within our team provides different perspectives on the problem and motivates innovative approaches for investigation,” said @RuochiZhang. ericandwendyschmidtcenter.org/updates/print-… @YanHu41082612 @JD_Buenrostro @broadinstitute @Nature

Gene regulation is complex, with ~2,000 TFs interacting with ~1M CREs. @YanHu41082612, Max Horlbeck (@JD_Buenrostro), @RuochiZhang (@Schmidt_Center) unveil PRINT & seq2PRINT: tools decoding TF/CRE binding at single-cell resolution. @broadinstitute @Nature ericandwendyschmidtcenter.org/updates/print-…

Nature research paper: Multiscale footprints reveal the organization of cis-regulatory elements go.nature.com/40jyD88

Excited to share our latest work, where we develop TDAC-seq to simultaneously map CRISPR-induced genetic variants & chromatin accessibility at targeted genomic loci by leveraging a dsDNA cytidine deaminase, DddA. Highlights below: 1/11 biorxiv.org/content/10.110…

Super excited to share our new study from the @JD_Buenrostro Lab in @Nature! We developed a computational method for tracking transcription factor and nucleosome binding using single-cell ATAC-seq and deep learning. Paper: nature.com/articles/s4158…

Thrilled to see our ExIGS tool featured! A true testament to the power of collaboration. Huge congrats to the team for their hard work! 🧬@ajaylabade31 @z_chiang @CarolineComenho nature.com/articles/d4158…

Introducing IN SITU GENOME SEQUENCING, a new technology to resolve DNA sequence🧬 and structure🔬 A team effort with @Andrew_C_Payne, @PaulReginato, @eboyden3, @JD_Buenrostro, and Fei Chen science.sciencemag.org/content/early/… This is how it works: (1/8)

This is phenomenal!! ExIGS: Expansion in situ Genome Sequencing!! —> Enabling genomic DNA sequencing and superresolution localization of nuclear proteins in single cells!! By @z_chiang @ajaylabade31 @JD_Buenrostro & co biorxiv.org/content/10.110…

7 years ago, I met a junior fellow named @JD_Buenrostro who blew me away with a vision of futuristic genomic technologies Today, we (@ajaylabade31, @carolinecomenho) are excited to share our first steps into that future: Expansion in situ genome sequencing 1/

So excited to finally share ExIGS, a new tech we (@z_chiang @ajaylabade31 @JD_Buenrostro) developed to combine spatial genomics with expansion microscopy