Ilya Osterman
@IlyaOsterman
Senior Research Associate, @SorekLab, @WeizmannScience, the phage-host arms-race
Our NAD-reconstitution story is finally published in @nature rdcu.be/dU5AQ Thank you to all co-authors, especially to @hadarsamra, @FrancoisRousset, Elena Loseva, @ErezTzvi, @AdiMillman, and everybody in the @SorekLab for their help and support.
"Little pigeons can carry great messages🕊️" Phages produce NAD+ by one-step reactions from nicotinamide (all other organisms need at least two) and protect themselves against multiple bacterial immune systems. Check out our new preprint - biorxiv.org/content/10.110… A thread below⬇️
Excited to share our latest collab with Wenyuan Han's Lab! 🚨 Bacteria use dITP as an immune signal to activate NAD+-depleting effectors. Phages counter immunity by destroying dAMP (dITP precursor)! 💥🧬 Preprint 🔗: biorxiv.org/content/10.110…
We cracked the tRNA specificity of FaRel2 toxSAS. A fun collaboration with the @SuzukiRNALab, Garcia-Pino @gem__atkinson and Polikanov. Out in @ScienceAdvances! science.org/doi/10.1126/sc…
📢Preprint out! Excited to share my final work from the @Soreklab! We mined phage dark matter using structural features shared by anti-defense proteins (viral tools that help phages bypass bacterial immunity) to guide discovery. Found 3 new families targeting immune signaling!
CRISPR continues to amaze me! Excited to share the last project from my PhD, co-led by the incredible @PujaMajumder91 and myself! We studied the CRISPR effector Cat1, which forms beautiful filament networks to degrade NAD+. Out today in @ScienceMagazine! science.org/doi/10.1126/sc…
And here it is, the completed BASEL collection finally published as early access in @PLOSBiology - see the thread for more details. 🤗🧬🎣
🚨 🧬 Updated preprint on the completed BASEL collection - what's new? Some fun with bacterial immunity and a new P22 relative infecting E. coli K-12. A thread 🧵
Another cADPR isomer involved in bacterial immunity has been discovered. For me, N7 was the least expected position; I was sure it would be N3, but no... So, there are two more positions for cyclization that should be discovered: N3 and -NH2. More Thoeris systems are waiting! :)
Now published @ScienceMagazine: TIR signaling activates caspase-like immunity in bacteria We report a new immune molecule: N7-cADPR. Produced by phage-induced TIRs and activates a defensive bacterial caspase Congrats @FrancoisRousset & @IlyaOsterman! science.org/doi/10.1126/sc…
Very happy our paper on CARD domains in gasdermin anti-phage defense systems is now published in @Nature 🥳 Thanks to all the people involved especially the Kranzusch lab and @SorekLab nature.com/articles/s4158…
Our paper out @Nature: CARD domains mediate anti-phage defense in bacterial gasdermin systems CARDs are essential for caspase recruitment in inflammasome activation. We now find them in bacterial immune systems Congrats @TanaWein! Thanks Kranzusch lab! tinyurl.com/CA88DD
Our paper out @CellCellPress: Structure-guided discovery of viral proteins that inhibit host immunity Congrats @ErezTzvi, Azita Leavitt, @amitai_gil, our collaborators @ Kranzusch lab, and coauthors A 🧵 1/10 cell.com/cell/abstract/…
I'm thrilled to announce our latest work is now published in Cell! Viruses encode numerous proteins that inhibit host defenses, but identifying immune-modulatory proteins among millions of viral sequences has been nearly impossible - until now! cell.com/cell/fulltext/…
Very happy to share our new review in @MolecularCell covering nucleic acid recognition and effector mechanisms for various prokaryotic immune systems! Was a great experience creating this with @MarraffiniLab! cell.com/molecular-cell…
Thank you to Nathan P. Bullen and @aaronwhiteley for the beautiful review of our NARPs paper.
Phages gain the upper hand in the metabolic arms race for NAD+ dlvr.it/TGh0qy
1/11 - With @KMitosch and Clément Potel + colleagues in @savitski_lab @TypasLab @embl, I’m very happy to share our discovery of a hyper-promiscuous phage protein kinase (T7K) which broadly deactivates bacterial defenses! biorxiv.org/content/10.110…
Very happy our paper on Zorya anti-phage defense system is now published in @Nature! 🥳 Big congrats to first authors @HaidaiHu and @__jphilipp and all other authors! Wonderful collab with @Salmo_Lab and @SimonJacksonNZ #cryoEM @UCPH_CPR @koebenhavns_uni nature.com/articles/s4158…
We are excited to share our latest work on the cooperation between RNA polymerase and the ribosome in bacteria: science.org/doi/10.1126/sc… The work was supported by @IGBMC, @Inserm, @CNRS, @unistra, @ERC_research, @IMC_Bio, and @instructhub. 1/7
Get ready to dance, our paper – Phage DisCo: targeted discovery of bacteriophages by co-culture – has been pre-printed! 🪩 This has been a really fun project to work on with @implosian, @baym, @nquinoneso, Kesther and Carmen! biorxiv.org/content/10.110… 1/
Thank you to @lundbeckfonden, @novonordiskfond & @ERC_Research for the support!
It has been a journey and here it is: my first post doc paper is out in Nature Communications 🎉🎉🎉 If you red the preprint, it's worth checking the revised manuscript, we added some cool stuff. A thread. ⬇️ nature.com/articles/s4146…
Thanks to @ArturoCarabias & Guillermo Montoya (@g1_guillermo) for the beautiful review of our discovery and especially for the very nice and clear summary figure - I will use it a lot😀
🔦 Spotlight! @ArturoCarabias & Guillermo Montoya (@g1_guillermo) highlight a recent study in @Nature by Rotem Sorek (@SorekLab) and colleagues describing 2 ways phages can reconstitute NAD+ to counter bacterial immunity dlvr.it/TGJtS7
Fancy a postdoc in Munich? For a collaborative project between @SorekLab and @hornung_lab on antiviral innate immunity, we are looking for exceptional candidates with skills in immunology / biochemistry / computational biology. More details here: genzentrum.uni-muenchen.de/career/postdoc…