Harshini Chandrashekar

I am excited to share our new work from @CreminsLab on genome’s structure-function relationship in post-mitotic human neurons and a neurological disease context (biorxiv.org/content/10.110…)! (1/n)

@titusk99 with colleagues @HarshiniChandr8 @simandi_zoltan found a link among de novo enhancer-promoter loops, RNApol2 elongation, and robust gene expression upregulation during human neural lineage commitment. Collab @PaquetLab Now published: doi.org/10.1016/j.xgen…

Excited to see this out in print! 🎉🧬

This has been a team effort across the Cremins lab! Special shoutout to @KennethHPham for helping in crafting and iterating FISHnet, and @HarshiniChandr8 for helping with genomic analyses. (7/8)

Rory McIlory entered the final round of this year's Masters tournament in a position to win. After 11 years of trying, it would mean a career grand slam, placing him on the Mount Rushmore of Golf's all-time greats. He was looking good—until hole 13, when he made a terrible…

Congratulations @HarshiniChandr8 💐 Using human neurons homozygously-engineered with familial AD mutations, she found a role for miswired promoter-enhancer multi-loops in dysregulated excitatory and inhibitory gene expression programs BioRxiv: doi.org/10.1101/2024.0…

Congratulations to Rohan Patel @PatelRohanD: Awarded an NIH fellowship for his work to computationally explore chromatin patterns and mechanisms of loop extrusion in sequential oligopaints imaging data. doi.org/10.1101/2024.0…

Excited and honored to be a finalist for the @GenomeOrgAus #YoungTADAward! 🎉 Thank you for the opportunity and looking forward to sharing our work from @CreminsLab @PennEngineers @pennbioeng ! 🧬✨

First preprint from the lab 🚨 How can enhancers regulate target promoters across vast genomic distances, and what is the role of cohesin loop extrusion in the process? biorxiv.org/content/10.110… (1)

I am thrilled to share that I've successfully defended my Ph.D. from @CreminsLab @pennbioeng @PennEngineers ! The big day was made more special with my family and friends present to support me! 💫

Harshini has done a terrific job during her time in the lab - it has been wonderful to watch her grow and refine her special qualities throughout her PhD:

Finally, I'd like to give thanks to @simandi_zoltan, @HarshiniChandr8, and @PaquetLab for their critical wet and dry lab contributions and @CreminsLab for guiding me through the scientific discovery and writing process. Without them, none of this would've been possible! (5/5)

Women are born with all their oocytes, which need to stay viable for decades to ensure fertility. How are they maintained for that long? Our latest research in @NatureCellBio reveals that oocyte maintenance involves exceptional protein longevity. nature.com/articles/s4155… (1/9)

Next up is MASTR-seq - a protocol for Cas9-based capture of target loci for multi-modal inquiry of short tandem repeat tracts and DNA methylation at single-allele resolution using Nanopore long read sequencing. Led by @chuanbin_su

check out Rohan's FISHnet for sequential Oligopaint data, so lucky to have been a part of Rohan's project

FISHnet is a graph theory method that capture hierarchical chromatin domains using 4 key steps. (2/8)