Wiedenheft Lab
@WiedenheftLab
News & views from Wiedenheft lab at Montana State University @montanastate | Account is managed by lab members.
I am delighted to share the newest work from the @SternbergLab, in which we discover RNA-templated gene creation as a mechanism of antiviral immunity. This is a story for anyone who loves molecular biology, and one that repeatedly left us in disbelief. doi.org/10.1101/2024.0…
Selfish genes rock!!! Click on the link to find out why. Thanks to all my coauthors for being incredible mentors. @WiedenheftLab @HarmitMalik @janet_young_123 @artemnemudryi @ANemudraia oup2-idp.sams-sigma.com/authorize?auth…
New paper from the Wiedenheft lab! #CRISPR #RNA
Cellular repair of DNA breaks enables editing of the human genome. Do human cells repair RNA breaks, and can we use it to edit transcriptomes? Our work with @ANemudraia and @WiedenheftLab is now online in @ScienceMagazine! #ScienceResearch science.org/doi/10.1126/sc…
I'm excited to announce the start of my group @HoerLab @Helmholtz_HIRI in beautiful Würzburg! We'll study RNA phages, focusing on bacterial immunity and regulation 🦠🧬 Want to join us? Send me an email or find me at the conference in Boston next week!
My lab is almost set up, and I'm looking for great people to join it! I am currently recruiting a research technician to drive an exciting project on molecular antagonism between bacteria and phage. If you're interested, DM me or apply to this position: wd1.myworkdaysite.com/recruiting/upe…
Incredible seminar visit to @montanastate this week thanks to my generous host @henriques_will (grad student in @WiedenheftLab that I’ve had privilege of co-mentoring past 4 years). First of all, Bozeman airport is one of the prettiest airports I’ve flown into
Interested in #CRISPR? Check out this postdoc opening @SantiagoFrangos lab!
📢I'm hiring a Postdoc to join me at Dept. of @PennBiology at @Penn! We'll use bioinformatics 🖥️, biochem 🧪, and cryo-EM ❄️🔬, to study how CRISPR systems work & applications. Work on cool science with a supportive mentor! Reposts appreciated!
Truly great work from @iamamardeeep on the structure and function of the PARIS defense system!
In this bioRxiv preprint, we (@_KevinCorbett ) present the architecture and activation mechanism of the PARIS antiviral defense system. Thanks to our co-authors, @Lisa_Liang13, @EnustunEray, and @PoglianoJoe, for their support in shedding light on this Anti-anti-defense system.🧵
Ringing in the new year with a pre-print on the PARIS defense system! This work defines how viral proteins activate PARIS-mediated tRNA cleavage and how viral tRNAs rescue infection. tinyurl.com/5x7epzxd. Also check out complementary work from the Corbett lab!
New pre-print from the Sternberg Lab! Transposon-encoded nucleases (TnpB/IscB) gave rise to the CRISPR-associated proteins (Cas9 and Cas12), but were TnpB-like proteins domesticated for other functions? 𝙔𝙀𝙎!! doi.org/10.1101/2023.1…
I'm excited to share our findings on a metal-dependent specificity switch for #CRISPR-Cas12a! We profiled the specificity of Cas12a at four Mg2+ concentrations and observed striking differences in mismatch tolerance at lower, physiological conditions 1/n biorxiv.org/content/10.110…
Out today in @MolMicroEditors: Inhibition of PQS signaling by the Pf bacteriophage protein PfsE enhances viral replication in P aeruginosa doi.org/10.1111/mmi.15… With our 🇨🇦 friends @DezielEric and @theMaxwellLab
Research at MSU has explored CRISPR immune systems and their role in DNA manipulation. In a new publication from researchers in @MSU_MicroImmuno, that technology is applied to RNA using brand new methods. montana.edu/news/23165/
Montana State scientists publish research on CRISPR DNA bending montana.edu/news/23181/mon…
Thrilled to see our latest work published this week, on the essential roles of IscB/TnpB -- ancestral homologs of Cas9/Cas12 -- in transposon maintenance & spread. A wonderful team effort led by the talented Chance Meers! Thread on bioRxiv version below... nature.com/articles/s4158…
Great news - our lab's latest manuscript is out on bioRxiv! Led by postdoc Chance Meers, we discovered that RNA-guided DNA cleavage by transposon-encoded nucleases arose to facilitate the pervasive spread and inheritance of transposons. 🧵: (1/6) biorxiv.org/content/10.110…
Our CRISPR-based RNA virus engineering study was covered in the latest #TWiV 1045 episode 🎙️🦠 Thanks Vincent @profvrr, @RichCondit, Brianne @BioProfBarker, Alan, and Angela @ImmuneVet for a very thoughtful discussion of our work!
TWiV 1045: Less Lassa, CRISPR RNA viruses 〰️ TWiV reviews a Lassa virus mRNA vaccine that confers protection against disease without inducing neutralizing antibodies, and a CRISPR-based method for engineering the genome of RNA viruses. 📺 bit.ly/3PEvpYO
I’m excited to share the preprint of my first first-author paper! The pandemic was a challenging time to start my PhD, but it spawned the awesome opportunity to join a second lab 800 miles west in Seattle to study ancient viruses in the human genome! 🧵👇
Now out in NSMB! How genome folding directs site-specific integration of phage DNA fragments into the CRISPR locus! Read here: rdcu.be/dmfSC Many thanks to co-authors for this collective effort! Thanks to @kellogg_liz and other reviewers for their thoughtful feedback!
Vertebrates, bacteria, and archaea have domesticated transposases for adaptive immunity. Transposases often get help from other DNA-bending proteins. But what’s the role of DNA bending in the cutting and pasting of DNA? biorxiv.org/content/10.110… (1/16)
Our study is on the cover! 😍
New research establishes a recombinant #RNA technology that can rapidly engineer RNA viruses for applications in antiviral therapeutics, vaccine design, and screening mutations for phenotypes. Learn more in this week’s issue of Science Advances: scim.ag/453
Our latest work with @ANemudraia and @WiedenheftLab is now available @bioRxiv! We show that #CRISPR-guided RNA breaks are repaired in human cells, and this RNA repair enables site-specific RNA editing. See the thread👇🏽 biorxiv.org/content/10.110…