Freddie King
@Freddie_King00
PhD candidate in Bozkurt Lab @ Imperial. Synthetic biology 🤝 plant immunity
I finally got around to publishing my MPMI poster on 'Developing broad bean as a new model system for studying plant immunity'! We show that you can do HR assays, transient RNAi, and confocal microscopy in this legume species🌱🫘Check it out: zenodo.org/records/1631... #2025MPMI
Excited to share our work at Windbichler lab on shufflon invertases where we demonstrate an assay for testing Rci-like recombinases! A couple of them even outperform Rci pubs.acs.org/doi/full/10.10… @nikiwind #synbio
Mind the GAP🚆⚠️- Excited to share my latest in @ScienceAdvances! We discovered a pathogen's novel traffic control mechanism🦠🚦 - targeting a plant protein to remodel the pathogen interface and suppress defense 🌱 #cellbiology #plantbiology #TOPGAP doi.org/10.1126/sciadv…
Final version of our manuscript reporting how a plant pathogen hacks host membrane trafficking is out. Great team work with @dromius @BubeckLab and @KamounLab science.org/doi/10.1126/sc… 😊👇
Mind the GAP-New research alert!🚨Our latest study revealed the first known case of a pathogen effector targeting a RabGAP! 🦠The effector remodels the pathogen interface and suppresses defense-related secretion mediated by its cognate Rab.🌱🚦 #research biorxiv.org/content/10.110…
Hot off the press! A great colab with the @Tolga_Bzkrt team led by @EnochYuen - feat. the P. palmivora effector TIKI (trafficking interference & tissue-killing) and its target TOPGAP (a RabGAP) studied by @edouard_evan & @TulinFrej. science.org/doi/10.1126/sc…
Final version of our manuscript reporting how a plant pathogen hacks host membrane trafficking is out. Great team work with @dromius @BubeckLab and @KamounLab science.org/doi/10.1126/sc… 😊👇
A pathogen effector co-opts a host RabGAP protein to remodel pathogen interface and subvert defense-related secretion | Science Advances science.org/doi/10.1126/sc…
Part of my postdoctoral work in the Ronald lab is on bioRxiv! We applied directed evolution to engineer a new-to-nature immune recognition range in a rice immune receptor
Directed evolution of a plant immune receptor for broad spectrum recognition of pathogen effectors biorxiv.org/cgi/content/sh… #biorxiv_plants
1/7 🧵Excited to share this work! Let me walk you through the phylogenomics and motif analysis of CCR-NLRs to add to @TarhanEI thread. Plus, stick around for a cool #OpenPlantNLR dataset at the end!
🚨 A new twist in #NLRbiology! 🚨 Excited to report on the surprising mechanism where the helper NLR NRG1 targets organellar membranes to trigger immunity unlike canonical CC-NLRs, which function at the plasma membrane! 1/11 🧵 doi.org/10.1101/2024.0…
⚠️🚨Exciting new preprint🚨⚠️ We report that the helper NLR protein NRG1 targets organellar membranes to trigger immunity!🔬🌱 Awesome collaboration between the labs of @Tolga_Bzkrt, @KamounLab, @jonathandgjones, @AdamWu9527, @JiorgosKourelis! doi.org/10.1101/2024.0…
🚨 A new twist in #NLRbiology! 🚨 Excited to report on the surprising mechanism where the helper NLR NRG1 targets organellar membranes to trigger immunity unlike canonical CC-NLRs, which function at the plasma membrane! 1/11 🧵 doi.org/10.1101/2024.0…
A helper NLR targets organellar membranes to trigger immunity biorxiv.org/cgi/content/sh… #biorxiv_plants
Thrilled to see this out! Rational immune receptor engineering and proof-of-concept transferring expanded perception. Big thanks to @JerryTianrunLi for leading experiments, with support from Esteban Bolanos, Hanxu Sha, @Dani_M_Stevens, & @daniilprigozhin. biorxiv.org/content/10.110…
A new preprint from @kseniakrasileva lab: Engineering pathogen-inducible promoters for conferring disease resistance in tomato biorxiv.org/content/10.110…
Great example of AFM application from the van der Hoorn lab, as always. Also check out our manuscript from February 2023, where we demonstrate the accuracy of AFM in cross-kingdom interactions. journals.plos.org/plosbiology/ar…
Using AlphaFold Multimer to discover interkingdom protein–protein interactions onlinelibrary.wiley.com/doi/10.1111/tp… @ThePlantJournal
A new preprint from @kseniakrasileva lab: resurrecting an NLR to recognize its effector escape mutant!
Engineering the plant intracellular immune receptor Sr50 to restore recognition of the AvrSr50 escape mutant biorxiv.org/cgi/content/sh… #biorxiv_plants
I am *beyond thrilled* to have the work from my PhD finally out!! 💫 We found that MLA3, a plant immune receptor, mimics a virulence target of Magnaporthe oryzae to set a trap for this fungus and trigger disease resistance. How cool is that?! 🤓 🧵1/15 biorxiv.org/content/10.110…