FPbase

Try the new FPbase spectra viewer! Completely rewritten to be faster, more customizable, and full-featured: • SVG/PNG/CSV export • state recovery & URL sharing • keyboard shortcuts for rapid entry 💚 • efficiency calculations • full documentation fpbase.org/spectra

Hi Fluorescence friends! Please follow future updates on Bluesky: bsky.app/profile/fpbase…

This exercise was less informative than I'd hoped, but perhaps it's interesting to someone else: spectroscopically-inferred estimates of densities of states for a selection of fluorescent proteins spanning the visible spectrum. Data from @FPbase.

recently hit 2,000,000 sessions on @FPbase ! 📈

Mutagenesis of #FbFP #LOV domain: 🟠 22 variants with 486-512 nm emission maxima 🟠 three-channel imaging via spectral unmixing 🟠 two-channel fluorescence lifetime unmixing doi.org/10.1101/2022.1… Congratulations, @ivangushchin, @Aynya5, and all the authors! @FPbase

Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s4159…

Chapter on using FPbase tools out now in the MMB issue on FPs. Thank you for editing @mayankdu!

this is genius: @helsinkiuni BioImaging Unit utilizes @FPbase to allow users test their fluorophores fpbase.org/microscope/u4g… cc @Daniel_Wa19

anyone out there routinely express FPs in Chlamydomonas? any modern suggestions for SNR? (presumably red, but any expression gotchas?)

🥁 Delighted to announce that our FPCountR paper is now on bioRxiv! In this paper we describe the development of a method to allow absolute fluorescent protein quantification using microplate readers for synthetic circuit characterisation. biorxiv.org/content/10.110… 🧵

Always nice to see features put to use! @eszter_cs and @gbstan use the FPbase API (among other things) in a method for the calibration of fluorescence readings on microplate readers; for quantification of FP concentration biorxiv.org/content/10.110…

Just discovered the interactive chart of FPbase.com. Wow, what an amazing tool to compare different properties across a wide range of fluorescent proteins. Great work @TalleyJLambert 🙂 fpbase.org/chart/

FPbase is amazing! I created scope configurations for @UAZCancer core instruments, and link to it with a little QR code posted next to the scope! And made a nice lab exercise for my microscopy class. iScopeCalc rocks too. Keep ‘em coming, @TalleyJLambert!

This is beautiful and makes me want to rewrite FPbase using it 😍

Dear imaging companies, Please tell your customers (or make available on request) the *actual* spectra for all components (LEDs, filters, etc) in their light path. just saying "460-490nm" is not sufficient. It's amazing how many scientists can't get this info from their reps 😞

a reminder on the difficulty in comparing fluorescence photobleaching measurements across studies (and even across probes on the same scope) fpbase.org/bleaching/

📣 Apply for an award from Addgene to attend a conference! We're accepting applications until April 30 ☑️ Undergrad, grad student, postdoc, research tech ☑️ Working w/ fluorescent proteins ☑️ Planning to attend a conference, virtual, hybrid, or in-person hubs.ly/H0LbWNd0

Databases are hard... and no amount of automated error checking can catch every mistake that humans make (in the literature or in submissions). FPbase gratefully accepts help/corrections from anyone! Here's an example of what not to do if you find something needing correction 😂

more JF spectra than you can shake a stick at. (2P coming soon) thanks @rhodamine110! fpbase.org/spectra/?s=637…