ESC & iPSC News

LOF mutations in the PINK1 & PRKN genes are the most common cause of early-onset #ParkinsonsDisease. A @MayoClinicNeuro study from the Springer lab explored PRKN-activating mutations as a potential therapeutic avenue, finding a temporary gain of activity: bit.ly/4nG0GJX

Non-human primates offer a promising avenue for developing genetically matched ESCs for regenerative medicine. 🐒 Researchers derived three live monkeys and their genetically matched autologous ESCs using embryo splitting. Get the full story: go.nature.com/4kqCNmR

Dr. Anna Bigas' team presents a novel and unbiased approach to identifying endogenous components to specify hematopoietic stem cells from #PSCs. 🧫 Learn more in @BloodPortfolio! 👇 💡 bit.ly/3H1wRDb

Researchers report a unique metabolic method to promote #hPSC-cardiomyocyte differentiation that involves marked suppression of the mitochondrial oxidative phosphorylation from the mesendoderm to the cardiac mesoderm. bit.ly/455zwnv

Among the most recurrent epigenetic defects in #PSCs are errors in genomic imprinting. 🧬 The authors provide an overview of imprinting defects in both mouse and human PSCs, delving into their origins and consequences. bit.ly/4obqazh

Scientists report a unique metabolic method to promote #hPSC-cardiomyocyte differentiation. They show that serine synthesis pathway can regulate cardiomyocyte lineage specification. | @iScience_CP bit.ly/455zwnv

Scientists report a heparan sulfate mimetic containing a DNA aptamer with affinity for the membrane-associated #pluripotency marker, alkaline phosphatase, that can be selectively targeted to the surface of #ESCs. 🔗 bit.ly/3IX1nyv

The latest research out of @MPI_MolGen clarifies the genomic targets and role of HELLS in human cells. 🎯 Drs. Alexander Meissner, Helene Kretzmer, and team generate HELLS and DNMT3A/B knockout hPSCs. 🔗 bit.ly/4kSoiIJ

Dr. Mitzi Kuroda and colleagues dissect the molecular mechanisms underlying aberrant function of BCL-6 corepressor (BCOR)-internal tandem duplication (ITD) mutant by employing #mESCs. 📓 Learn more about their protocol: bit.ly/44Phi9t

Introducing: A #pluripotent stem cell atlas of multilineage differentiation! 🧫 Dr. Palpant's team generated a scRNA-seq time course to study the role of developmental signaling pathways on multilineage diversification from pluripotency in vitro. 🔗 go.nature.com/4nTxpLX

Scientists from Sanquin Research Amsterdam describe the optimization of an #iPSC to RBC differentiation platform and its step-by-step translation process to dynamic culture conditions. 🧫🩸Check it out: bit.ly/4nQKIN2

Porcine extended potential stem cells (pEPSCs) exhibit both self-renewal and #pluripotency. This work uncovers a role of FOXH1 involved in the core regulatory network that orchestrates gene expression programs to maintain pluripotency of pEPSCs. 🔗 bit.ly/44NzPTN

This review summarizes different sources of human somatic cells used for the generation of #iPSCs, namely blood cells, keratinocytes, urine-derived epithelial cells, and mesenchymal stem cells. 📖 Give it a read: bit.ly/44H5c21

DOT1L, which plays a role in leukemia pathogenesis, decorates actively transcribed genes with H3K79 methylation. Emmalee Cooke & Cheng Zeng (@cwru) have helped target DOT1L mutation-driven diseases by uncovering the downstream effects of methylation loss: bit.ly/46Dg1ov

The Fiorenzano lab (@Fiorenzano555)'s new review on human midbrain organoid development is a guaranteed dopamine release for anyone trying to model the dopaminergic system. 🧠 See the paper in @embojournal to learn about advancements in #BrainOrganoids: bit.ly/4eEW6Yi

#ESCs display a distinctive resistance against various viruses, but the mechanism behind this is unclear. In this study from @PKU1898, researchers identified VAMP5 as a potent cell-autonomous defense factor against coronaviruses: go.nature.com/3GzObyO

Scientists in the Pathak lab (@phataks) have their eyes on #PIEZO1. 👀 🔬 They genetically engineered hiPSCs to express a HaloTag fused to endogenous PIEZO1, opening up new ways to study PIEZO1 #mechanotransduction in human systems: go.nature.com/3GnBqYf

By integrating multi-omics data across #ESC states, scientists including Dr. Wange Lu identified super-enhancers (SE) interacting with master transcription factors, exemplified by the Klf5-adjacent SE. 🧬 📚 Read it in @NatureComms: go.nature.com/4kqHQng

This @NatureComms paper highlights #iPSC-derived mature epicardium’s potential in investigating adult epicardial reactivation, pivotal for effective cardiac regeneration. This study was led by Dr. Yoshida and collaborators at @KyotoU_News! 🙌 go.nature.com/4kjOWde

Read about jawbones in a dish in this new @Nature study. 🧫 Scientists from Kyoto University present a method for generating jawbone-like #organoids from #hiPSCs through PA1 ectomesenchyme of the mandibular prominence. go.nature.com/4ewsD2P