DenardResearchGroup
@CarlDenard
Assist. Prof. of Chem. Eng. Enzyme Therapeutics, Synthetic Biology, Protein Engineering and Design. http://www.thedenardlab.com Soccer player. Music lover.
Online now @ Cell is the yeast multicellular engineering paper from @DrFankangMeng - the fruits of his productive PhD. He developed modular synthetic biology tools to bring multicellular behaviours to yeast - adhesion, juxtacrine signalling and more. cell.com/cell/fulltext/…
TwitterWorld, my lab wants to get into some optogenetics work (bacteria, yeast, in vitro). Are there good protocols to build cheap systems out there?
Excited to share the Hanson Lab’s @ADHansonLab new preprint! We used OrthoRep to evolve Arabidopsis HDH, a short-lived enzyme, selecting variants with up to 20× higher abundance. Mutations boosted lifespan, catalytic efficiency, or inhibitor resistance. 🔗doi.org/10.1101/2025.0…
Has anyone made RNA-triggered toehold switches that work in S. cerevisiae? If you know of some good papers, let me know.
"New" Paper Alert from a collab with Jang Lab. We designed and characterized globular protein vesicles with ability to sense small molecules in their surroundings. Congrats to her awesome students. pubs.acs.org/doi/abs/10.102…
Happy to share an authoritative, topical, and detailed review paper on cover all the latest efforts to engineer proteases, out now in Biotech Avdances. sciencedirect.com/science/articl… Led by @sammartinusen, with considerable efforts from many lab members.
Streptavidin beads, two years expired from Mitenyi Biotech. Should we still use for MACS?
In reading Chang Liu's paper on using serine integrases to increase transformation efficiencies in the OrthoRep P1 plasmid, could not help but wonder. Why aren't there more library DNA transformations performed in yeast using integrases rather than homologous recombination?