Alonso-Stepanova Lab

New article from the lab, in collaboration Olivier Loudet et al. We show that increasing recombination ~6-fold empowers the detection and resolution of QTLs, reaching the gene scale with only a few hundred plants. nature.com/articles/s4200…

Development of this new genetic tool was sparked by a spontaneous discussion at a scientific conference. One two-year NSF EAGER grant to jump-start the work and 5 more years of "side projects" by 18 talented scientists made EBSn possible. biorxiv.org/content/10.110…

Mario's vitamin C study uncovers an interesting link between this essential metabolite and the regulation of local auxin biosynthesis biorxiv.org/content/10.110…

Katie Vollen's PhD prelim exam writeup transformed into a nice review article on large DNA manipulation: Way to go, Katie! Beyond a few bases: methods for large DNA insertion and gene targeting in plants - Vollen - 2025 - The Plant Journal - onlinelibrary.wiley.com/doi/full/10.11…

𝐅𝐨𝐜𝐮𝐬𝐞𝐝 𝐫𝐞𝐯𝐢𝐞𝐰📚 Beyond a few bases: methods for large DNA insertion and gene targeting in plants🧬🌱 📖doi.org/10.1111/tpj.70… 🖊️Katie Vollen @ANStepanova45 @AlonsoStepanova 🗺️@NCState Large DNA insertions in plants are challenging, but new tools improve efficiency

If you're struggling to assemble large DNA constructs, check out Chengsong's DASH system, which combines Golden Gate cloning, recombineering, and site-specific recombinases to build multigene constructs of ~100kb. biorxiv.org/content/10.110…

Happy to have been able to contribute to the implementation of this exciting new genome editing tool coming out of the Chase Biesel lab! @BeiselLab Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes biorxiv.org/content/10.110…

🌿 Today, we remember Salk Professor Joanne Chory, a visionary plant biologist and a force for climate action. Her research revolutionized how we understand plant biology and inspired solutions to fight climate change. Joanne’s brilliant mind and passion will forever impact the…

📢CALL FOR REVIEWERS📢We´re expanding our reviewer community 🌍 onlinelibrary.wiley.com/journal/136531… 🌱Interested in joining? email [email protected] 🍀New to peer review? Wiley offers a free online course 👉wileyresearcheracademy.com/p/all-you-need… TPJ supports co-reviewing with senior mentor

5/5 🌍 Written with the hope of inspiring children worldwide to explore the fascinating world of plant science and research. Let's cultivate the next generation of plant enthusiasts together! a.co/d/faC1Tr4 #PlantScience #ArbiTheModelPlant #PlantPostdocs #ASBP

Looking forward to this massive reunion of Ecker lab alumni 🌱🧬🧠

Wonderful video! Big congratulations to Salk Professor Chory!

Excited to share Matt's preprint on Golden Braid-based tools! These innovative methods enhance geminivirus vectors for high-level protein expression and provide repair templates for gene targeting in plants. Check it out and let us know your thoughts. biorxiv.org/content/10.110…

I ordered @ATinyGreenCell's chromoprotein palette to do some agar art + science outreach. But when my lab saw the rainbow plates, we decided it would be fun to see if we could express the purple chromoprotein TsPurple in lettuce. Long story short, it worked :D #LavenderLettuce

Our new CRISPRi-based synthetic gene circuit platform for programmable, reversible control of gene expression in plants 🌱 @NatureBiotech. A compact and modular logic gate system with useful new capabilities for plant engineering nature.com/articles/s4158…

Please save the date for IPGSA 2025 to be held in Ft. Collins, CO from June 29 - July 3, 2025.