good papers

"genome shuffling by protoplast fusion between genetically diverse Bacillus strains generates frequent, unbiased, genome-wide recombination that mimics the effects of sexual recombination. ... we leveraged protoplast fusion to establish a bacterial QTL mapping platform"

The extended mobility of plasmids "conjugative transfer and plasmid-encoded advantages can sustain plasmid prevalence despite the plasmid paradox. Microbiome engineering is shifting to precision interventions at the strain, plasmid, or gene level." academic.oup.com/nar/article/53…

New review article with @MichaelMDesai is out today! Grateful for the opportunity to contribute something we hope will serve the community well

Excited to share our labs latest paper. We use #synbio for biosensor discovery to aid our quest to build 'smart probiotics' to sense and treat disease. Well done @KlondikeClare et al! Thanks @wellcometrust for funding! embopress.org/doi/full/10.10… @ImperialInfect @TheCrick

Construction and phenotypic classification of synthetic dual-pole Escherichia coli cells "a genetic circuit that enables two distinct scaffolding proteins to localize at opposite poles in E. coli" nature.com/articles/s4200…

Beyond nature's clock: Accelerating genomic diversity through hypermutation Targeted mutagenesis technologies sciencedirect.com/science/articl…

"there was little overlap between the substitutions found by directed evolution of the RamR transcription factor and those predicted by ML-based tools. ML-designed protein variants tested proved not to be functional."

Escherichia coli selection strains for growth-coupled metabolic engineering "growth-coupled selection brings synthetic metabolism to life" sciencedirect.com/science/articl…

Wearing bacteriophages individually with an adhesive drug-loadable nanohelmet for treating ocular infections "drug-loaded helmeted phages as eye drops for treating ocular infections" science.org/doi/10.1126/sc…

Advanced research and exploration of CRISPR technology in the field of directed evolution sciencedirect.com/science/articl…

Morphology engineering facilitates constructing efficient cell factories "... review the morphology regulation mechanisms in key industrial microorganisms to improve product yields" sciencedirect.com/science/articl…

Flow-induced 2D nanomaterials intercalated aligned bacterial cellulose "For our bioreactor configuration and a growth period of 10 days, the dry cellulose sheet yield was determined to be ~7.5 mg/day." nature.com/articles/s4146…

Engineered Lactiplantibacillus plantarum WCFS1 as a Biosensor Probe for Lung Cancer "engineered biosensor detects proteases secreted by lung tumors" pubs.acs.org/doi/10.1021/ac…

Droplet microfluidics–based detection of rare antibiotic-resistant subpopulations in Escherichia coli from bloodstream infections "The growth of rare resistant cells is detected by observing droplet shrinkage" science.org/doi/10.1126/sc…

"a Cas9 targeted sequencing assay to selectively enrich and sequence genomic regions of interest" Context-Seq: CRISPR-Cas9 targeted nanopore sequencing for transmission dynamics of antimicrobial resistance nature.com/articles/s4146…

"in vivo MutaT7 mutagenesis-mediated screening pipeline to evolve oxygen-tolerant rubisco in E. coli" In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance pnas.org/doi/10.1073/pn…

Data-driven protease engineering by DNA-recording and epistasis-aware machine learning "A recombinase-based DNA recorder that tracks protease activity against multiple substrates with high-throughput barcode sequencing" nature.com/articles/s4146…

🗺️ 🤖 “Technology Roadmap of Micro/Nanorobots” review has been published in @acsnano. This MEGA Review—authored by 103 researchers 🧑‍🔬 , spanning 161 📃 A4 pages, and citing 1,765 references—brings together leading groups from around the world. See pubs.acs.org/doi/10.1021/ac…

Engineering modular enzyme assembly: synthetic interface strategies for natural products biosynthesis applications pubs.rsc.org/en/content/art…

Programmable genome engineering and gene modifications for plant biodesign sciencedirect.com/science/articl…