John F Marko

@JohnFMarko

Professor, Molecular Biosciences and Physics & Astronomy, Northwestern University 🙂🐭🐸🦎🔬📏

Evanston, Illinois, USA

Joined April 2013

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721Followers

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John F Marko@JohnFMarko · Oct 14, 2020

doi.org/10.1093/nar/gk… DNA plectoneme pinning by base pair mismatches - thanks Keir et al. for a great collaboration!

JohnFMarko's tweet card. Abstract. Damaged or mismatched DNA bases result in the formation of physical defects in double-stranded DNA. In vivo, defects in DNA must be rapidly and e

John F Marko@JohnFMarko · Jul 16

Enjoy Dr. Parth Desai's recent study of the suppression of DNA strand separation by molecular crowding pubmed.ncbi.nlm.nih.gov/40312912/ #biophysics #singlemolecule #DNA

JohnFMarko's tweet image. Enjoy Dr. Parth Desai's recent study of the suppression of DNA strand separation by molecular crowding
pubmed.ncbi.nlm.nih.gov/40312912/
#biophysics #singlemolecule #DNA

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John F Marko@JohnFMarko · May 13

Commemorate the life and science of our dear departed colleague Jon Widom - this Thurs May 15 1230 pm in Pancoe Auditorium @NUmolbiosci @NorthwesternU - our distinguished Widom Lecturer is Prof. Lucy Bai @penn_state - Chromatin Accessibility and the 3D Genome

JohnFMarko's tweet image. Commemorate the life and science of our dear departed colleague Jon Widom - this Thurs May 15 1230 pm in Pancoe Auditorium @NUmolbiosci @NorthwesternU - our distinguished Widom Lecturer is Prof. Lucy Bai @penn_state - Chromatin Accessibility and the 3D Genome

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John F Marko@JohnFMarko · May 5

Why did the US government decide to start funding science and training young scientists? Worth reading this answer written in 1945 - for the moment still posted on the @NSF website at nsf-gov-resources.nsf.gov/2023-04/Endles…

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John F Marko@JohnFMarko · Apr 24

Subchromosomal mechanics of pericentric and centromeric chromatin along stretched mitotic chromosomes - surprise is little effect of CENP-N and -C degradation on CENP-A organization pubmed.ncbi.nlm.nih.gov/39937678/ From Ron Biggs and @StraightLab #centromere #kinetochore #chromosome

JohnFMarko's tweet image. Subchromosomal mechanics of pericentric and centromeric chromatin along stretched mitotic chromosomes - surprise is little effect of CENP-N and -C degradation on CENP-A organization
pubmed.ncbi.nlm.nih.gov/39937678/
From Ron Biggs and @StraightLab
#centromere #kinetochore #chromosome

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John F Marko@JohnFMarko · Apr 14

In case anyone is left out there, I'm pleased to report that Dr. Ning Liu's paper on mechanics of meitotic metaphase chromosomes, dependence on #cohesin #aging and #toxins was just published in eLife elifesciences.org/articles/97403 Enjoy!

JohnFMarko's tweet image. In case anyone is left out there, I'm pleased to report that Dr. Ning Liu's paper on mechanics of meitotic metaphase chromosomes, dependence on #cohesin #aging and #toxins was just published in eLife elifesciences.org/articles/97403 Enjoy!

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John F Marko@JohnFMarko · Jul 22, 2024

Just returned from the Topoisomeases in Biology and Medicine meeting case.fiu.edu/news-events/to… Thanks so much to organizers Yuk-Ching Tse-Dinh, Dagmar Klostermeier, Valerie Lamour @valeroche and John Nitiss for running a great meeting!

JohnFMarko's tweet image. Just returned from the Topoisomeases in Biology and Medicine meeting case.fiu.edu/news-events/to… Thanks so much to organizers Yuk-Ching Tse-Dinh, Dagmar Klostermeier, Valerie Lamour @valeroche and John Nitiss for running a great meeting!

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John F Marko@JohnFMarko · Jul 22, 2024

To avoid piles of corporate nonsense in every new tab in Edge I embarked on a quest to have new tabs load a user-defined URL. Apparently possible only by adding a 3rd-party browser extension, e.g., microsoftedge.microsoft.com/addons/detail/… It works but I'm sure the victory is only temporary.

JohnFMarko's tweet image. To avoid piles of corporate nonsense in every new tab in Edge I embarked on a quest to have new tabs load a user-defined URL. Apparently possible only by adding a 3rd-party browser extension, e.g., microsoftedge.microsoft.com/addons/detail/… It works but I'm sure the victory is only temporary.

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John F Marko@JohnFMarko · May 7, 2024

Dr. Ron Biggs has set up a really great #YouTube library of #chromosome manipulation movies plus some lab classics - including detailed video protocols. Let us know if you find them useful! youtube.com/channel/UC980J…

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John F Marko Retweeted

Evanston, IL Police@EvanstonPD · May 19, 2023

Cop on a Rooftop is in full effect at Dunkin located 1900 Dempster. We’re here until noon. Stop by and help support the Special Olympics and our local athletes! #CopOnARooftop

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John F Marko@JohnFMarko · Mar 28, 2023

Ban assault weapons now. sign.moveon.org/petitions/ban-…

JohnFMarko's tweet card. Senate Democrats have introduced the "Assault Weapons Ban of 2025," a bill that would reinstate a nationwide ban on military-style assault weapons by prohibiting the sale, transfer, manufacture,...

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John F Marko@JohnFMarko · Jul 30, 2022

Amidst a lot of noise about our fair nation becoming a "geriatric oligarchy" I blundered across this 2014 academic paper cambridge.org/core/journals/… Things have improved since 2014, right?

John F Marko@JohnFMarko · Apr 27, 2022

Published! Stefanos Nomidis' MD simulations of SMC complexes translocating and loop-extruding according to the model of Diebold-Durand et al. (Mol. Cell. 2017) academic.oup.com/nar/advance-ar…

JohnFMarko's tweet card. Abstract. Structural Maintenance of Chromosomes (SMC) complexes play essential roles in genome organization across all domains of life. To determine how th

John F Marko@JohnFMarko · Feb 25, 2022

I am recruiting a postdoctoral researcher (grant-funded position available now) interested in DNA-protein interactions and genome organization, using single-molecule techniques including magnetic tweezers and fluorescence approaches. markolab.bmbcb.northwestern.edu/marko/position…

John F Marko@JohnFMarko · Feb 9, 2022

Revised preprint - Stefanos Nimodis' MD simulations of the loop-capture model of SMC complexes biorxiv.org/content/10.110… DNA in the tripartite ring and broken symmetry of the complex itself permit processive translocation via large (up to 200 nm) steps

John F Marko@JohnFMarko · Jun 23, 2021

Now in @eLife - how the much-loved nuclear protein HP1α controls nuclear shape and mechanics, stiffens mitotic chromosomes, and boosts mitotic reliability, via dimerization+chromatin cross-bridging: elifesciences.org/articles/63972 - a project facilitated by @4DNucleome and @NCIPhySci

John F Marko Retweeted

Enrico Carlon@CarlonEnrico · Mar 16, 2021

"DNA tension-modulated translocation and loop extrusion by SMC complexes revealed by molecular dynamics simulations" disq.us/t/3vkncij with S. Nomidis, @Gruber_Lausanne and @JohnFMarko

John F Marko@JohnFMarko · Dec 14, 2020

ATP-dependent and supercoil-sensing DNA compaction by SMC5/6 - thanks to the D'Amours lab for involving us in this exciting project! pubmed.ncbi.nlm.nih.gov/33301731/

JohnFMarko's tweet card. The structural organization of chromosomes is a crucial feature that defines the functional state of genes and genomes. The extent of structural changes experienced by genomes of eukaryotic cells can...