Jian Li

@Jian_Li_SHT

PhD@TU Berlin, PostDoc@Northwestern, PI@ShanghaiTech University. Cell-free synthetic biology, Natural product biosynthesis.

Shanghai, CN

Joined March 2020

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138Followers

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Jian Li@Jian_Li_SHT · Apr 10

Establishing a High-Yield Bacillus subtilis-Based Cell-Free Protein Synthesis System for In Vitro Prototyping and Natural Product Biosynthesis | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…

Jian_Li_SHT's tweet card. Cell-free systems are emerging as powerful platforms for synthetic biology with widespread applications in both fundamental research, such as artificial cell construction, and practical uses like...

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Jian Li@Jian_Li_SHT · Apr 28

Our new work -- Integrase enables synthetic intercellular logic via bacterial conjugation | Cell Systems @babuxiang @ShanghaiTechUni cell.com/cell-systems/f…

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Jian Li@Jian_Li_SHT · Apr 3

Establishing a Serine Integrase‐Based Genetic Memory System In Vitro - Biotechnology and Bioengineering …iencejournals.onlinelibrary.wiley.com/doi/full/10.10…

Jian_Li_SHT's tweet card. This study establishes an in vitro genetic memory system using orthogonal serine integrases to integrate DNA information within a composite matrix. This platform enables biotechnology applications,...

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Jian Li@Jian_Li_SHT · Mar 20

Happy to share our new work in @NatureComms ! AI-driven high-throughput droplet screening of cell-free gene expression (doi.org/10.1038/s41467…). Hope our DropAI platform can accelerate the development of cell-free expression systems. @ShanghaiTechUni

Jian_Li_SHT's tweet card. Nature Communications - Cell-free gene expression (CFE) systems are often constrained by numerous biochemical components required to maintain biocatalytic efficiency. Here, the authors propose a...

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Jian Li@Jian_Li_SHT · Feb 9

Biocatalytic Reductive Amination for In Vitro Biosynthesis of the Amaryllidaceae Alkaloid Precursor - ChemCatChem - Wiley Online Library …mistry-europe.onlinelibrary.wiley.com/doi/10.1002/cc…

Jian_Li_SHT's tweet card. 4′-O-Methylnorbelladine (4′-MB) is a key precursor for the synthesis of Amaryllidaceae plant-derived alkaloids. In this study, an efficient biosynthetic system for the production of 4′-MB was...

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Jian Li Retweeted

Chemistry Europe@ChemEurope · Dec 6

#ChemPlusChem Enzyme-particle Complexes Facilitate Pickering Interfacial Biocatalysis (Jian Li and co-workers) • onlinelibrary.wiley.com/doi/10.1002/cp…

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Jian Li@Jian_Li_SHT · Dec 3

Chitin-Functionalized Cell-Free System Enables Sustainable Biocatalysis and Gene Expression | ACS Sustainable Chemistry & Engineering pubs.acs.org/doi/10.1021/ac…

Jian_Li_SHT's tweet card. Cell-free protein synthesis (CFPS) has proven invaluable for expressing a wide array of proteins and enzymes, boasting significant advantages, such as facile manipulation, rapid mass transfer, and...

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Jian Li Retweeted

bioRxiv Synthetic Biology@bioRxiv_synbio · Oct 2

Cell extract-based in vitro DNA replication enables sustainable cell-free gene expression biorxiv.org/cgi/content/sh… #biorxiv_synbio

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Jian Li@Jian_Li_SHT · Sep 6

Orthogonal Serine Integrases Enable Scalable Gene Storage Cascades in Bacterial Genome | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…

Jian_Li_SHT's tweet card. Genome integration enables host organisms to stably carry heterologous DNA messages, introducing new genotypes and phenotypes for expanded applications. While several genome integration approaches...

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Jian Li@Jian_Li_SHT · Aug 11

Using cell-free system to help understand the biosynthetic mechanism in natural product biosynthesis -- An Enzymatic Oxidation Cascade Converts δ-Thiolactone Anthracene to Anthraquinone in the Biosynthesis of Anthraquinone-Fused Enediynes | @JACS_Au pubs.acs.org/doi/10.1021/ja…

Jian_Li_SHT's tweet card. Anthraquinone-fused enediynes are anticancer natural products featuring a DNA-intercalating anthraquinone moiety. Despite recent insights into anthraquinone-fused enediyne (AQE) biosynthesis, the...

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Jian Li@Jian_Li_SHT · Jul 27, 2024

Officially tenured and promoted to Associate Professor. So grateful to my group members, mentors, collaborators, friends, and my family for their incredible support!

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Jian Li Retweeted

Synthetic Biology and Engineering - SCIEPublish@Syn_Bio_Eng · Jun 13, 2024

#LatestPaper #Plasmid_assembly #SyntheticBiology Welcome to read the #HighlyCited article: Serine Integrase-based Recombination Enables Direct Plasmid Assembly In Vivo by Dr. Jian Li et al. Enjoy reading🔗 sciepublish.com/article/pii/99

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Jian Li@Jian_Li_SHT · May 22, 2024

Very happy to share our new work -- A cell-free gene expression-based unified biocatalysis (UniBioCat) system for biosynthesis, engineering, and discovery of RiPPs natural products. @MichaelCJewett @ShanghaiTechUni link.springer.com/10.1038/s41467…

Jian_Li_SHT's tweet card. Nature Communications - Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a major class of natural products with potent biological activities but a vast majority of...

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Jian Li@Jian_Li_SHT · May 4, 2024

An In Vitro Hybrid Biocatalytic System Enabled by a Combination of Surface-Displayed, Purified, and Cell-Free Expressed Enzymes | ACS Synthetic Biology pubs.acs.org/doi/10.1021/ac…

Jian_Li_SHT's tweet card. Enzymatic cascades have become a green and sustainable approach for the synthesis of valuable chemicals and pharmaceuticals. Using sequential enzymes to construct a multienzyme complex is an effect...

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Jian Li@Jian_Li_SHT · Mar 10, 2024

Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis onlinelibrary.wiley.com/doi/10.1002/an…