Felipe Opazo
@FelipeOpaz_o
PI at the @yourUMG, Funder, and #CTO of @_NanoTag_, using #nanobodies for #SuperResolution #microscopy in #neurosciences.
The next big episode is dropping on Monday! Here is a short snippet. :) #optogenetics #expansion #microscopy
How to manipulate the brain with light? Optogenetics with Ed Boyden Our next guest on Neuroscience and Beyond is Prof. Dr. Ed Boyden, a pioneering neuroscientist who has transformed the neurotechnology with revolutionary methods like optogenetics, which enables precise control…
On October 24th, NanoTag´s CSO (Dr. Steffen Frey) will give a seminar on how our technologies 🦙 are reshaping how scientists do #immunofluorescence. If you are in Milano 🇮🇹, join @SanRaffaeleMI on the #ALEMBIC facility; otherwise, you can join live by following the QR code below
Dear remaining friends on Twitter, I won't check this account regularly anymore. The amount of garbage content has become intolerable. You can find me and scientific news in🦋Blue Sky: @opazo.bsky.social
💙On #RareDiseaseDay, we highlight the power of scientific innovation in #RareDisease research. 🔬Our #FluoTag®-X2 anti-mEos contributed to a study on Severe Startle Disease, revealing that GlyRβ enables mutGlyRα1 targeting but fails to restore function. 📄doi.org/10.1523/jneuro…
Thrilled to share our latest review paper, "Advanced Fluorescence Lifetime-Enhanced Multiplexed Nanoscopy of Cells", co-authored with @samub28, now published in Methods in Microscopy! 🚀✨ Check it out here: degruyter.com/document/doi/1… #FLIM #SMLM #FLPAINT #FLSMLM #MIETPAINT
#FluorescenceFriday is perfect for launching a new 🦙sdAbs (#nanobodies): FluoTag® conjugated to Janelia Fluor® 635b, a new buffer-independent self-blinking dye. Now You find FluoTag®-X2 anti-ALFA, anit-GFP & anit-mEos: nano-tag.com/shop/
Last paper of 2024! Excellent collaboration with @petrikursula, @ZucheroLab, and Hauke Werner´s lab. We characterized a set of 🦙#nanobodies binding myelin protein CNPase. It is now online at @JNeurochem here: onlinelibrary.wiley.com/doi/10.1111/jn…

Effective controls are essential. That’s why we’re excited to launch 𝐏𝐞𝐧𝐭𝐚𝐓𝐚𝐠 a versatile, positive control protein featuring five widely used epitope tags: #FLAG, #HA, #MBP, #Myc, and #ALFA. Check it out here 👉 bit.ly/415MWzb
𝐀𝐫𝐞 𝐲𝐨𝐮 𝐥𝐨𝐨𝐤𝐢𝐧𝐠 𝐟𝐨𝐫 𝐭𝐨𝐨𝐥𝐬 𝐭𝐨 𝐜𝐡𝐚𝐫𝐚𝐜𝐭𝐞𝐫𝐢𝐳𝐞 𝐲𝐨𝐮𝐫 #GFP-𝐭𝐚𝐠𝐠𝐞𝐝 𝐩𝐫𝐨𝐭𝐞𝐢𝐧? 💚 Discover our selection of anti-GFP🦙sdAb-based products here: bit.ly/3YTH1e2, and let us know how we can support your research!
Hello all! After years in the non-social wilderness, I am on X. And for a good reason! To announce the Göttingen 2025 #ExM Forum, #GEF25, a celebration of 10 years of expansion microscopy. Organized together with @eboyden3, @LabSauer & @AliHShaib
Hej, Stockholm! We are at #BioEurope (@EBDgroup) in 🇸🇪! We are on-site to showcase our 𝐬𝐢𝐧𝐠𝐥𝐞-𝐝𝐨𝐦𝐚𝐢𝐧 𝐚𝐧𝐭𝐢𝐛𝐨𝐝𝐲 🦙 technologies & discovery services for diagnostic, therapeutic, and drug development. Stop by booth #72 to discuss your project with our CEO and CSO
Thrilled to share the most comprehensive Atlas of Proteome/Phosphoproteome Turnover across mouse tissues and brain regions, thanks to the outstanding @Yanshen73854711 & @JunminPengLab! Proud to contribute to this. Check it out: #Proteomics #ProteinTurnover biorxiv.org/content/10.110…
Great technique!
It is out! #NanoPlex is the universal strategy for multiplexing any immunofluorescence using erasable #nanobodies from @_NanoTag_. We showcase easy multiplexed STED, STORM, and 21-plex Confocal #Microscopy. Published in @NatureComms here: rdcu.be/dWvzO
Exciting moment at the Single Molecule Workshop 2024 by @PicoQuant! @RomanTsukanov took the stage by storm with cutting-edge insights on microfluidics-based multiplexed DNA-PAINT, unlocking new potential for ultra-precise molecular imaging.
For microscope aficionados: #NanoPlex stain > image > erase > repeat @FelipeOpaz_o and co. present this great new set of methods for iterative imaging using secondary nanobodies Full nature.com/articles/s4146… @focalplane_jcs #microscopy
😯😯
It is out! #NanoPlex is the universal strategy for multiplexing any immunofluorescence using erasable #nanobodies from @_NanoTag_. We showcase easy multiplexed STED, STORM, and 21-plex Confocal #Microscopy. Published in @NatureComms here: rdcu.be/dWvzO
Absolutely Fantastic work by Felipe and the team..! Cheers.
It is out! #NanoPlex is the universal strategy for multiplexing any immunofluorescence using erasable #nanobodies from @_NanoTag_. We showcase easy multiplexed STED, STORM, and 21-plex Confocal #Microscopy. Published in @NatureComms here: rdcu.be/dWvzO
Congratulations for this amazing work! 🎉🎉🎉 Multiplexing at its best! 🔥 I was more than happy to contribute!